Reporter Gene Imaging: Methods and Protocols: Methods in Molecular Biology, cartea 1790
Editat de Purnima Dubeyen Limba Engleză Hardback – 2 iun 2018
Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații
ISBN-13: 9781493978588
ISBN-10: 1493978586
Pagini: 215
Ilustrații: XI, 221 p. 53 illus., 42 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.63 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 1493978586
Pagini: 215
Ilustrații: XI, 221 p. 53 illus., 42 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.63 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Cuprins
1. Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function
Ajit Dhadve, Bhushan Thakur, and Pritha Ray
2. Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging
Ajit Dhadve, Bhushan Thakur, and Pritha Ray
3. Optical Bioluminescence Protocol for Imaging Mice
David Stout and John David
4. Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase
Anne E. Gibbons, Kathryn E. Luker, and Gary D. Luker
5. Reporter Based BRET Sensors for Measuring Biological Functions In Vivo
Maitreyi Rathod, Arijit Mal, and Abhijit De
6. Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains
Ying Kong and Jeffrey Cirillo
7. In vivo Bacterial Imaging using Bioluminescence
Mariette Barbier, Justin Bevere, and F. Heath Damron
8. Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging
Jukka Pajarinen, Tzu-hua Lin, and Stuart B. Goodman
9. Hypoxia-induced Reporter Genes with Different Half-lives
Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, and Zaver M. Bhujwalla
10. Visualization of Immune System Reconstitution by Bioluminescent Imaging
Purnima Dubey
11. Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo
Melissa M. McCracken
12. Ex vivo Radiolabeling and In Vivo PET Imaging of T Cells Expressing Nuclear Reporter Genes
Maxim A. Moroz, Pat Zanzonico, Jason T. Lee, and Vladimir Ponomarev
13. Imaging T Cell Dynamics and Function by PET using Human Nuclear Reporter Genes
Jason T. Lee, Maxim A. Moroz, and Vladimir Ponomarev
14. Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT
Zimin Chen and Jianghong Rao
15. Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes
Nikunj B. Bhatt, Darpan N. Pandya, William A. Dezarn, Frank C. Marini, Dawen Zhao, William H. Gmeiner, Pierre L. Triozzi, and Thaddeus J. Wadas
16. Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models
Jessica Swanner and Ravi Singh
Ajit Dhadve, Bhushan Thakur, and Pritha Ray
2. Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging
Ajit Dhadve, Bhushan Thakur, and Pritha Ray
3. Optical Bioluminescence Protocol for Imaging Mice
David Stout and John David
4. Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase
Anne E. Gibbons, Kathryn E. Luker, and Gary D. Luker
5. Reporter Based BRET Sensors for Measuring Biological Functions In Vivo
Maitreyi Rathod, Arijit Mal, and Abhijit De
6. Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains
Ying Kong and Jeffrey Cirillo
7. In vivo Bacterial Imaging using Bioluminescence
Mariette Barbier, Justin Bevere, and F. Heath Damron
8. Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging
Jukka Pajarinen, Tzu-hua Lin, and Stuart B. Goodman
9. Hypoxia-induced Reporter Genes with Different Half-lives
Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, and Zaver M. Bhujwalla
10. Visualization of Immune System Reconstitution by Bioluminescent Imaging
Purnima Dubey
11. Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo
Melissa M. McCracken
12. Ex vivo Radiolabeling and In Vivo PET Imaging of T Cells Expressing Nuclear Reporter Genes
Maxim A. Moroz, Pat Zanzonico, Jason T. Lee, and Vladimir Ponomarev
13. Imaging T Cell Dynamics and Function by PET using Human Nuclear Reporter Genes
Jason T. Lee, Maxim A. Moroz, and Vladimir Ponomarev
14. Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT
Zimin Chen and Jianghong Rao
15. Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes
Nikunj B. Bhatt, Darpan N. Pandya, William A. Dezarn, Frank C. Marini, Dawen Zhao, William H. Gmeiner, Pierre L. Triozzi, and Thaddeus J. Wadas
16. Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models
Jessica Swanner and Ravi Singh
Textul de pe ultima copertă
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Caracteristici
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts